François-Yves BOUGET

François-Yves BOUGET              

Directeur de Recherche 1ère classe CNRS-HDR

UMR7621 Laboratory of Microbial Oceanography, Banyuls sur Mer. France

Career highlights

• 1995 PhD University of North Carolina at Chapel Hill (NC, USA)
• 1996 EMBO Post-doctoral fellowship. Marine Biological Association Plymouth (UK).
• 1997 CNRS Permanent “Chargé de Recherche” position. Station Biologique Roscoff (France)
• 2001-2002 Sabbatical University of Ghent (Belgium).
• 2003-2009 Group Leader of the Circadian clock and cell division ATIP/ATIP+ young  investigator CNRS group, Banyuls sur Mer (France)
• 2006  Appointed as « Directeur de Recherche » CNRS.
• 2010-2013 present Group leader of the  “Systems Biology of environmental responses” team
• 2014- present. Responsible of the axis “Regulation of microbial function by light and nutriments”.
• 2015 Directeur de Recherche  1ère classe CNRS.

 Current research interests/projects

• Use functional genomics approaches in microbial organisms to understand how they respond to environmental forcing, in particular under diurnal conditions
• Determine the importance of circadian clock and photoperiodism in the ecological success of marine phytoplankton
• Promote model microorganisms for studies in oceanography, ecology, ecotoxicology
• Valorise microlgal biomass

Research funding since 2008

• 2020-2024: ANR CLIMA-CLOCK. Photoperiodism in ubiquist model microalgae of marine phytoplankton: mechanisms and sensitivity to ocean temperature increase (PI).
• 2019-2021: Gordon and Betty Moore Foundation. A molecular toolbox for Bathycoccus prasinos and Ostreococcus lucimarinus (PI)
• 2015-2019 : ANR Defi1 Adaptation au Changement climatique. « PhotoPhyto » , Effect of global warming on the spring bloom initiation, Photoperiodism, Composition, adaptation (PI).
• 2017-2021: H2020 European project  « ASSEMBLE+ » . Development of genetic resources in picoeukaryotes.
• 2018-2021 : ANR «CINNAMON»   Crosscale analysis of adaptation to iron limitation in a key microorganism.
• 2016-2017: Gordon and Betty Moore Foundation. Development of genetic transformation in phytoplanktonic picoeukaryotes (PI).
• 2017-2018: Gordon and Betty Moore Foundation. Toward stable genetic transformation in phytoplanktonic picoeukaryotes (PI).
• 2014-2017. ANR Labcom. “OTARI”. Ostreococcus tauri from research to Industry (PI).
• 2015-2018. INFRADEV4 European project.  European Marine Biological Research Infrastructure Cluster to promote the Blue Bioeconomy (Partner).
• 2012-2015: ANR Blanche « Phytoiron » (Iron metabolism in marine phytoplankton)
• 2012-2017: Investissement d’Avenir EMBRC France. Coordinator of JDA2 (Genetic resources). “Insertion mutant collection in Ostreococcus”.
• 2009-2013: European project  « ASSEMBLE »  (responsible of genetic resources WP)
• 2008-2011: ANR/BBSRC Systems Biology « Miniclock » (coordinator). Modelling the circadian clock in a minimal model organism.

Publications and patents

1. Lambert S, Lozano JC, Bouget FY*, Galand P* (*co-corresponding authors). Seasonal marine microorganisms change neighbors under remarkable weather conditions. Environmental Microbiology (revised manuscript).
2. Faktorová D, Nisbet R.E, Fernández Robledo JA ... Bouget FY... Lukeš J. (2019). Genetic tool development in marine protists: Emerging model organisms for experimental cell biology. Nature Methods 17, 481–494 (2020). doi.org/10.1038/s41592-020-0796-x.
3. Koedooder C, Van Geersdaële R, Guéneuguès A, Bouget FY, Obernosterer I, Blain S (2020). The interplay between iron limitation, light and carbon in the proteorhodopsin-containing Photobacterium angustum S14. FEMS Microbiol Ecol.6(7):fiaa103. doi: 10.1093/femsec/fiaa103.
4. Annunziata R, Ritter A, Fortunato AE, Cheminant-Navarro S, Agiera N, Huysman M, Petroutsos D, Finazzi G, Wingeg P, Bones A, Bouget FY, Lagomarsino MG, Bouly JP, Falciatore A. (2019). RITMO1 regulates diurnal rhythmicity in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. pii: 201819660. doi: 10.1073/pnas.1819660116
5. Smith, D, Buddie, AG, Goss, R J M, Overmann, J, Lepleux, C, Brönstrup, M, Kloareg, B, Meiners, T, Brennecke, P, Ianora, A, Bouget, FY, Gribbon, P, Pina, M (2019). Discovery pipelines for marine resources – an ocean of opportunity for biotechnology? World Journal of Microbiology and Biotechnology World J Microbiol Biotechnol. 35(7):107. doi: 10.1007/s11274-019-2685-y.
6. Lambert S, Tragin M, Lozano JC, Ghiglione JF, Vaulot D, Bouget FY*, Galand PE* (2019). Rhythmicity of coastal marine picoeukaryotes, bacteria and archaea despite irregular environmental perturbations. ISME Journal, 13: 388–401 (*co-corresponding authors).
7. Paerl RW, Bertrand E, Schatt P, Mehiri, Niehaus TD, Hanson AD, Riemann, Bouget FY (2018). Carboxylated thiazole: A newly recognized microbial micronutrient currency and, critical component of thiamin biosynthesis. Nature Sci Rep Sci Rep. 2018 Jun 6;8(1):8876. doi: 10.1038/s41598−018−27042−8.
8. Henríquez−Castillo CA, Botebol H, Mouton A, Ramírez−Flandes S, Lozano JC, Lelandais G, Andrade S, Trefault N, De la Iglesia RA, Bouget FY (2018). Genetically engineered Ostreococcus tauri biosensors for copper pollution in coastal marine environments.Front. Environ. Sci., 22. doi.10.3389/fenvs.2018.00022.
9. Guyon JB, Vergé V, Schatt P, Lozano JC, Liennard M, Bouget  FY (2018). Comparative analysis of culture conditions for the optimization of carotenoid production in several strains of the picoeukaryote Ostreococcus. Mar. Drugs 16(3), 76; doi:10.3390/md16030076
10. Koedooder C., Guéneuguès A., Van Geersdaële R., Vergé V., Bouget F.Y., Labreuche Y., Obernosterer I., Blain S. (2018) The Role of the glyoxylate shunt in the acclimation to iron limitation in a marine heterotrophic bacteria Frontiers in Marine Sciences 8-11 (435) 2296-7745). Doi:10.3389/fmars.2018.00435
11. Hirth M, Liverani S, Mahlow S, Bouget FY, Pohnert G, Sasso S (2017). Metabolic profiling identifies trehalose as an abundant and diurnally fluctuating metabolite in the microalga Ostreococcus tauri. Metabolomics 13(6):68. doi: 10.1007/s11306-017-1203-1.
12. Botebol H, Lelandais G, Six C, Lesuisse E, Meng A, Bittner L, Lecrom S, Sutak R, Lozano JC, Schatt P,  Vergé V, Blain S,Bouget FY (2017). Acclimation of a low iron adapted Ostreococcus strain to iron limitation through cell biomass lowering. Scientific Report 7(1):327. doi: 10.1038/s41598-017-00216-6.
13. Brodie J, Steven G. Ball S, Bouget FY, Chan CX, De Clerck O, Cock M, Gachon C, Grossman AR, Mock T, Raven J, Saha M, Smith A, Vardi A, Yoon HS, Bhattacharya D. (2017). Biotic interactions as drivers of algal origin and evolution. New Phytologist 216, 670-681.
14. Paerl RW, Bouget FY, Lozano JC, Schatt P, Verge V, Palenik P, Azam F (2017). Use of plankton-derived vitamin B1 precursors, especially thiazole-related precursor, by key marine picoeukaryotic phytoplankton. ISME J. doi: 10.1038/ismej.2016.145.
15. Lelandais G,  Scheiber I,  Paz-Yepes J, Lozano JC, Botebol H, Pilátová J, Žárský V, Léger T, Blaiseau PL, Bowler C, Bouget FY, Camadro JM, Sutak R, Lesuisse (2016). Ostreococcus tauri is a new model green alga for studying iron metabolism in eukaryotic phytoplankton. E. BMC Genomics 319. doi: 10.1186/s12864-016-2666-6.
16. Botebol H, Lesuisse E, Sutak R, Six C, Lozano JC, Schatt P, Vergé V, Kirilovsky A, Morrissey J, Léger T, Camadro JC, Gueunegues A, Bowler C, Blain S, Bouget FY (2015). Central role for ferritin in the day/night regulation of iron homeostasis in marine phytoplankton. Proc Natl Acad Sci U S A. 112 14652-14657.
17. Thommen Q, Pfeuty B, Schatt P, Bijoux A, Bouget FY, Lefranc M (2015). Probing entrainment of Ostreococcus tauri circadian clock by green and blue light through a mathematical modeling approach. Front Genet 6:65. doi:10.3389/fgene.2015.00065.
18. Morrissey J, Sutak R, Paz-Yepes J, Tanaka A, Moustafa A, Veluchamy A, Thomas Y, Botebol H, Bouget FY, McQuaid JB, Tirichine L, Allen AE, Lesuisse E, Bowler C (2015). A novel protein, ubiquitous in marine phytoplankton, concentrates iron at the cell surface and facilitates uptake. Curr Biol. 25(3):364-371. doi: 10.1016/j.cub.2014.12.004. 1.
19. Blanc-Mathieu R, Verhelst B, Derelle E, Rombauts S, Bouget FY, Carré I, Château A, Eyre-Walker A, Grimsley N, Moreau H, Piégu B, Rivals E, Schackwitz W, Van de Peer Y, Piganeau G. (2014). An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies. BMC Genomics.15:1103. doi: 10.1186/1471-2164-15-1103.
20. Weirauch MT, Yang A, Albu M, Cote AG, Montenegro-Montero A, Drewe P, Najafabadi HS, Lambert SA, Mann I, Cook K, Zheng H, Goity A, van Bakel H, Lozano JC, Galli M, Lewsey MG, Huang E, Mukherjee T, Chen X, Reece-Hoyes JS, Govindarajan S, Shaulsky G, Walhout AJ, Bouget FY, Ratsch G, Larrondo LF, Ecker JR, Hughes TR.(2014) Determination and inference of eukaryotic transcription factor sequence specificity. Cell. 158(6):1431-43. doi: 10.1016/j.cell.2014.08.009.
21. Lozano JC, Schatt P, Botebol H, Vergé V, Lesuisse E, Blain S, Carré I, Bouget FY (2014). Efficient gene targeting and foreign DNA removal by homologous recombination in the picoeukaryote Ostreococcus. Plant J 78, 1073-1083. doi: 10.1111/tpj.12530.
22. Bouget FY, Lefranc M, Thommen Q, Pfeuty B, Lozano JC, Schatt P, Botebol H, Vergé V (2014). Transcriptional versus non-transcriptional clocks: A case study in Ostreococcus. Mar Genomics 14, doi: 10.1016/j.margen.2014.01.00.
23. Botebol H, Sutak R, Blaiseau PL, Bouget FY, Camadro JM, Lesuisse E (2014). Different iron sources to study thephysiology and biochemistry of iron metabolism in marine micro-algae. Biometals 27, 75-88.
24. van Ooijen G, Hindle M, Martin SF, Barrios−Llerena M, Sanchez F, Bouget FY, O'Neill JS, Le Bihan T, Millar AJ (2013). Functional analysis of Casein Kinase 1 in a minimal circadian system. PLoS One 8: e70021. doi: 10.1371/journal.pone.
25. Collén J, Porcel B, Carré W, Ball SG, Chaparro C, Tonon T, Barbeyron T, Michel G, Noel B, Valentin K, Elias M, Artiguenave F, Arun A, Aury JM, Barbosa−Neto JF, Bothwell JH, Bouget FY, Boyen C (2013). Genome structure and metabolic features in the red seaweed Chondrus crispus shed light on evolution of the Archaeplastida. Proc Natl Acad Sci U S A.  110, 5247−5252.
26. Sanchez-Ferandin, S, Leroy F, Bouget FY, Joux F (2013). A new, sensitive marine microalgal recombinant biosensor using luminescence monitoring for toxicity testing of antifouling biocides. Appl Environ Microbiol. 79, 631-8.
27. Sutak R, Botebol H, Blaiseau PL, Leger T, Bouget FY, Camadro JM, Lesuisse E. (2012). A comparative study of iron uptake mechanisms in marine micro-algae: iron binding at the cell surface is a critical step. Plant Physiol 160, 2271-2284.
28. van Ooijen G, Knox K, Kis K, Bouget FY, Millar A J (2012). Genomic transformation of the picoeukaryote Ostreococcus tauri. Journal of Visual Experimentation 65, e4074. doi: 10.3791/4074.
29. Thommen Q, Pfeuty B, Corellou F, Bouget FY, Lefranc M (2012). Robust and flexible response of Ostreococcus tauri circadian clock to light/dark cycles of varying photoperiod. FEBS J 279, 3432-3448 (IF 3.478).
30. Pfeuty B, Thommen Q,  Corellou F, Djouani-Tahri EB, Bouget FY, Lefranc M (2012). Circadian clocks in changing weather and seasons: Lessons from the picoalga Ostreococcus tauri. Bioessays 34, 781-790.
31. Troein C, Corellou F, Dixon L E, van Ooijen G, O'Neill J S, Bouget FY (co-corresponding author) and Millar A J (2011). Multiple light inputs to a simple clock circuit allow complex biological rhythms. Plant J 66, 375-385.
32. Sorokina O, Corellou F, Dauvillee D, Sorokin A, Goryanin I, Ball S, Bouget FY, Millar A J (2011). Microarray data can predict diurnal changes of starch content in the picoalga Ostreococcus. BMC Syst Biol 5, 36. doi: 10.1186/1752-0509-5-36.
33. O'Neill JS, van Ooijen G, Dixon LE, Troein C, Corellou F, Bouget FY, Reddy AB, Millar AJ (2011). Circadian rhythms persist without transcription in a eukaryote. Nature 469, 554-558
34. Djouani-Tahri el B, Sanchez F, Lozano JC, Bouget FY (2011). A phosphate-regulated promoter for fine-tuned and reversible overexpression in Ostreococcus: application to circadian clock functional analysis. PLoS One 6, doi: 10.1371/journal.pone.0028471.
35. Djouani-Tahri el B, Christie J M, Sanchez-Ferandin S, Sanchez F, Bouget FY (corresponding author) and Corellou F (2011). A eukaryotic LOV-histidine kinase with circadian clock function in the picoalga Ostreococcus. Plant J  65, 578-588.
36. Bouget FY (2011). A new type of non genetic eukaryotic circadian clock. Med Sci (Paris) 5, 481-483.  
    Thommen Q, Morant P E, Corellou F, Bouget FY, Lefranc M (2010). Robustness of circadian clocks to daylight fluctuations: hints from the picoeucaryote Ostreococcus. PLoS Comp Biol 11, e1000990. doi: 10.1371/journal.pcbi.1000990.
37. Thommen Q, Morant P E, Corellou F, Bouget FY, Lefranc M (2010). Robustness of circadian clocks to daylight fluctuations: hints from the picoeucaryote Ostreococcus. PLoS Comp Biol 11, e1000990. doi: 10.1371/journal.pcbi.1000990.
38. Moulager M, Corellou F, Verge V, Escande M-L, Bouget FY. (2010). Integration of light signals by the retinoblastoma pathway in the control of S phase entry in the picophytoplanktonic cell Ostreococcus. PLoS Genet 6, e1000957. doi:10.1371/journal.pgen.1000957.
39. Morant PE, Thommen Q, Pfeuty  B, Vandermoere C, Corellou F, Bouget FY, Lefranc M (2010). A robust two-gene oscillator at the core of Ostreococcus tauri circadian clock. Chaos 20, 045108. doi: 10.1063/1.3530118.
40. Monnier A, Liverani S, Bouvet R, Jesson B, Smith JQ, Mosser J, Corellou F, Bouget FY (2010). Orchestrated transcription of biological processes in the marine picoeukaryote Ostreococcus exposed to light/dark cycles. BMC Genomics doi: 10.1186/1471-2164-11-192.
41. Heijde M, Zabulon G, Corellou F, Ishikawa T, Brazard J, Usman A, Sanchez F, Plaza P, Martin M, Falciatore A, Todo T, Bouget FY, Bowler, C. Characterization of two members of the cryptochrome/photolyase family from Ostreococcus tauri provides insights into the origin and evolution of cryptochromes. Plant Cell Environ. 33, 1614-1426.
42. Djouani-Tahri E B, Motta J P, Bouget FY, Corellou F Insights into the regulation of the core clock component TOC1 in the green picoeukaryote Ostreococcus. Plant Signal Behav 5, 332-335.
    Corellou F, Schwartz C, Motta JP, Djouani-Tahri EB, Sanchez F, Bouget FY (2009). Clocks in the Green Lineage: Comparative Functional Analysis of the Circadian Architecture of the Picoeukaryote Ostreococcus. Plant Cell 21, 3436-3449 (F1000 high light).
43. Corellou F, Schwartz C, Motta JP, Djouani-Tahri EB, Sanchez F, Bouget FY (2009). Clocks in the Green Lineage: Comparative Functional Analysis of the Circadian Architecture of the Picoeukaryote Ostreococcus. Plant Cell 21, 3436-3449 (F1000 high light).
44. Bouget FY, Moulager M, Corellou F (2007). Circadian regulation of cell division. In “Cell division control in plants. Ed DPS Verma. Springer Verlag.
45. Moulager M, Monnier A, Jesson B, Bouvet R, Mosser J, Schwartz C, Garnier L, Corellou F, Bouget FY (2007). Light-dependent regulation of cell division in Ostreococcus: evidence for a major transcriptional input. Plant Physiol 144, 1360-1369.
46. Picault S, Corellou F, Schwartz C, Bouget FY (2007). Simulation multi-agent de réseaux génétiques: les rythmes circadiens d’Ostreococcus tauri . Actes des 15e Journées Francophones sur les Systèmes MultiAgents. Cépaduès éditions, pages 149–158.
47. Van Damme D, Coutuer S, de Rycke R, Bouget FY, Inze D, Geelen D (2006). Somatic cyctokinesis and pollen maturation in Arabidopsis depend on TPLATE, which has domains similar to coat proteins. Plant Cell 18, 3502-3518
48. Derelle E, Ferraz C, Rombault S….Bouget FY...Moreau H (2006). Genome Analysis of the smallest free-living eukaryote Ostreococcus tauri unveils unique genome heterogeneity. Proc Nat Acad Sci USA 103, 11647-11652.
49. Corellou F, Coehlo SMB, Bouget FY, Brownlee C (2005). Spatial re-organisation of cortical microtubules in vivo during polarisation and asymmetric division of Fucus zygotes. J Cell Science 118, 2723-2734
50. Corellou F, Camasses A, Ligat L, Peaucellier G, Bouget FY(2005). Atypical regulation of a green lineage specific B-type cyclin dependent kinase. Plant Physiol 138, 1627-1636.
51. Van Damme D, Bouget FY, Inze D, Geelen D (2004). Identification of cytokinesis related Arabidopsis proteins through GFP-tagging and fluorescence profiling. Plant J 3, 386-398.
52. Corellou F, Brownlee C, Meijer L, Kloareg B, Bouget FY (2001). The first cell cycle of the Fucus zygote parallels a somatic cell cycle. Plant Cell 13, 585-598
53. Corellou F, Brownlee C, Kloareg B, Bouget FY. (2001). Cell cycle-dependent control of polarised development by a PSTAIRE CDK-like protein in the Fucus zygote. Development 128, 4383-4392.
54. Brownlee C, Bouget FY, Corellou C (2001). Choosing sides:  Establishment of polarity in zygotes of fucoid algae. Sem Cell and Dev Biol 12, 345-351.
55. Bouget FY, Corellou C, Kropf DL (2001). Fucoid algae as model organisms for investigating early embryogenesis. Cah Biol Mar. 42, 101-107.
56. Corellou F, Potin P, Brownlee C, Kloareg B, Bouget FY (2000). Inhibition of zygotic polarity by protein tyrosine kinase inhibitors leads to an alteration of embryo pattern in Fucus. Dev Biol 219, 165-182.
57. Corellou F, Bisgrove S, Kropf DL, Meijer L, Kloareg B, Bouget FY (2000 ). A S/M DNA replication checkpoint prevents nuclear and cytosplasmic events of cell division, including centrosomal axis alignment and and inhibits activation of G2/M cyclin dependent kinase like proteins in fucoid zygotes. Development 12, 1651-1660.
58. Brownlee C, Bouget FY (1998). Polarity determination in Fucus: From zygote to multicellular embryo. Sem Cell and Dev Biol 9, 179-185.
59. Brownlee C, Berger F, Bouget FY. (1998). Signals involved in control of polarity, cell fate and developmental pattern in Fucus. Symp Soc Exp Biol 51, 33-41.
60. Bouget FY, Berger F, Brownlee C (1998). Position dependent control of cell fate in Fucus embryos: Role of intercellular communication. Development 125, 1999-2008.
61. Bouget FY, Gerttula S, Shaw SL, Quatrano RS (1996).  Localization of actin messenger-RNA during the establishment of cell polarity and early cell divisions in Fucus embryos. Plant Cell 8, 189-201.
62. Bouget FY, Kerbourch C, Liaud MF, de Goer SL, Quatrano RS, Cerff R, Kloareg B (1995). Structural features and phylogeny of the actin gene of Chondrus-crispus (gigartinales, rhodophyta)", Curr Genet 28 164-172.
63. Bouget FY, Gerttula S, Kloareg B, Quatrano RS (1995) A new, nonradioactive whole-mount in-situ hybridization protocol for Fucus (phaeophyta) embryos.  J. Phycol 31, 1027-1030.
64. Bouget FY, Gerttula S, Quatrano RS (1995) Spatial redistribution of polyA+ rnRNA during polarization of the Fucus zygote is dependent upon microfilaments Dev Biol 171, 258-261.
65. Liaud MF, Valentin C,  Martin W, Bouget FY, Kloareg B, Cerff R (1994). The evolutionary origin of red algae as deduced from the nuclear genes encoding cytosolic and chloroplast glyceraldehyde-3-phosphate dehydrogenases from Chondrus crispus. J Mol Evol 38,319-327.
66. Liaud MF, Valentin C, Brandt U, Bouget FY, Kloareg B,  Cerff R (1993). The gapdh gene system of the red alga Chondrus crispus - promotor structures, intron-exon organization, genomic complexity and differential expression of genes. Plant Mol Biol 23, 981-994.

Patent

Corellou F and Bouget FY (2008).  International patent no 60/900,46. Expression of polypetides from the nuclear genome of Ostreococcus sp.

Enveloppe SOLEAU

1. Enveloppe N° 517324 220914 : François-Yves Bouget, Jean-Claude lozano, Valérie Vergé et Philippe Schatt. « Procédé de fabrication de transgènes pour transformation de Ostreococcus tauri ».

2. Enveloppe N° 517325 220914. François-Yves Bouget, Jean-Claude lozano, Valérie Vergé et Philippe Schatt «  Procédé de transformation d'Ostreococcus tauri par electroporation ».

3. Enveloppe N°531412 200215. François-Yves Bouget, Philippe Schatt, Jean-Claude lozano et Valérie Vergé. « Culture Cellulaire et production d’exopolysaccharides chez Ostreococcus tauri ».